Risda Febrianti, Ratna Kusuma, Samsurianto Samsurianto

PENGARUH PEMBERIAN ZPT EKSTRAK KECAMBAH KACANG HIJAU (Vigna radiata L.) PADA MEDIA MURASHIGE & SKOOG (MS) TERHADAP PERTUMBUHAN PLANLET KANTUNG SEMAR (Nepenthes sp.) SECARA IN VITRO

Introduction

Pengaruh pemberian zpt ekstrak kecambah kacang hijau (vigna radiata l.) pada media murashige & skoog (ms) terhadap pertumbuhan planlet kantung semar (nepenthes sp.) secara in vitro. Pengaruh ZPT ekstrak kecambah kacang hijau pada media MS terhadap pertumbuhan planlet kantung semar (Nepenthes sp.) in vitro. Solusi konservasi tanaman langka melalui kultur jaringan.

Abstract

Kantung semar (Nepenthes sp.) merupakan salah satu tanaman hias yang sering dijumpai di kawasan hutan. Tanaman ini masuk Convention on International Trade of Endangered Species (CITES), di mana semua tanaman yang masuk dalam CITES dilarang untuk diperdagangkan karena dianggap hampir punah. Untuk itu perlu ditemukan alternatif penggadaan bibit dengan pemanfaatan bioteknologi seperti kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian zat pengatur tumbuh ekstrak kecambah kacang hijau pada media MS terhadap pertumbuhan planlet tanaman kantung semar secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yaitu Konsentrasi Ekstrak Kecambah Kacang Hijau yang terdiri dari empat taraf (0 mL/L, 20 mL/L, 40 mL/L, 60 mL/L) sehingga terdapat 4 perlakuan dengan 3 ulangan. Uji statistika yang digunakan berupa uji non parametrik Kruskal-Wallis dan uji lanjutan menggunakan uji Mann-Whitney, dengan taraf signifikasi 5%. Hasil penelitian menunjukkan bahwa pemberian ZPT ekstrak kecambah kacang hijau dengan konsentrasi 20 ml/L mempengaruhi penambahan rata-rata tinggi tanaman sebanyak 1 cm. rata-rata jumlah daun pada konsentrasi 40 ml/L berpengaruh signifikan dan berbeda nyata dengan rata-rata jumlah daun sebanyak 9,33 tunas, sedangkan rata-rata jumlah tunas sebanyak 10,33 tunas dan rata-rata waktu muncul tunas sebanyak 3,33 tunas.

Review

This study addresses a highly relevant issue concerning the conservation of *Nepenthes sp.*, an endangered carnivorous plant listed under CITES, by exploring *in vitro* propagation techniques. The use of tissue culture, a powerful biotechnological tool, for multiplying such valuable species is well-justified given the challenges of conventional propagation and the need for sustainable alternatives to mitigate extinction risks. The objective to investigate the efficacy of mung bean sprout extract (ZPT) as a natural and potentially cost-effective plant growth regulator within an MS medium for *Nepenthes* planlet growth is commendable, offering a practical approach to enhance propagation success. The research employed a Completely Randomized Design (CRD) with four concentrations of mung bean sprout extract (0, 20, 40, and 60 mL/L) across three replicates, providing a robust experimental setup for comparative analysis. Statistical analysis utilized non-parametric tests, Kruskal-Wallis and Mann-Whitney, which are appropriate given the nature of the data often encountered in biological experiments. Key findings indicate that specific concentrations of the extract significantly influenced various growth parameters. Notably, a concentration of 20 mL/L led to an average increase of 1 cm in plant height. Furthermore, 40 mL/L was significantly effective in increasing the average number of leaves to 9.33, while the average number of shoots reached 10.33. The abstract also mentions an average "time for shoot emergence" resulting in 3.33 shoots, though the phrasing here could benefit from clarification regarding the units for time. Overall, this study provides valuable insights into optimizing *Nepenthes sp.* propagation *in vitro* using natural growth regulators. The identification of optimal concentrations of mung bean sprout extract for enhancing plant height and leaf/shoot production is a significant step towards developing more efficient and sustainable propagation protocols for this endangered species. While the abstract's phrasing regarding "waktu muncul tunas sebanyak 3,33 tunas" is slightly ambiguous and could be clarified in the full paper, the core findings are clear and promising. Future research could further elucidate the specific active compounds within the mung bean sprout extract responsible for these effects and explore their application across different *Nepenthes* species or growth stages, thereby contributing substantially to conservation efforts.

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