Ahmad Yunus, Sri Hartati

Pengaruh Kinetin dan GA3 Terhadap Pertumbuhan Anggrek Hibrida Coelogyne Secara In Vitro

Introduction

Pengaruh kinetin dan ga3 terhadap pertumbuhan anggrek hibrida coelogyne secara in vitro. Teliti pengaruh Kinetin & GA3 pada pertumbuhan anggrek hibrida Coelogyne secara in vitro. Temukan konsentrasi optimal untuk perbanyakan massal dan hasil tunas/daun terbaik.

Abstract

Anggrek hitam memiliki nilai ornamental dan ekonomi yang tinggi karena munculnya varietas unggul yang dikembangkan melalui hibridisasi dan seleksi. Kultur jaringan menawarkan metode yang efisien untuk perbanyakan massal dari varietas hibrida tersebut. Penelitian ini bertujuan untuk mengetahui konsentrasi optimal Kinetin dan GA3 untuk perbanyakan in vitro subkultur anggrek hibrida Coelogyne. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan pola faktorial yang terdiri dari dua variabel perlakuan dan empat ulangan. Faktor pertama adalah konsentrasi Kinetin yang terdiri dari tiga taraf: 0 ppm, 0,5 ppm, dan 1 ppm. Faktor kedua adalah konsentrasi GA3 yang juga terdiri dari tiga taraf: 0 ppm, 0,5 ppm, dan 1 ppm, sehingga terdapat 9 kombinasi perlakuan. Karakteristik yang diamati meliputi tinggi tanaman, jumlah tunas, jumlah daun, jumlah akar, panjang akar, dan berat tanaman. Hasil penelitian menunjukkan bahwa kombinasi Kinetin 0,5 ppm dan GA3 1 ppm menghasilkan jumlah tunas terbanyak (11,20 tunas) dan jumlah daun terbanyak (12,38 helai), sementara kombinasi Kinetin 0,5 ppm dan GA3 1 ppm juga menghasilkan tinggi tanaman optimal (1,41 cm), jumlah akar (7,00 buah), dan panjang akar (0,61 cm).

Review

This manuscript, titled "Pengaruh Kinetin dan GA3 Terhadap Pertumbuhan Anggrek Hibrida Coelogyne Secara In Vitro," presents a highly relevant and timely investigation into the *in vitro* propagation of *Coelogyne* hybrid orchids. The objective of optimizing Kinetin and GA3 concentrations for subculture is crucial for the mass production of these high-value ornamental plants. The study's clear focus on identifying optimal growth regulator combinations to enhance proliferation and overall plant development is well-justified given the ornamental and economic significance of orchid hybrids. The experimental design, a factorial Completely Randomized Design (RAL), is appropriate for systematically evaluating the effects of the chosen hormone concentrations. A significant strength of this research lies in its clear and quantitative elucidation of the optimal hormone concentrations. The findings precisely indicate that a combination of Kinetin 0.5 ppm and GA3 1 ppm yielded the most prolific shoot and leaf development, with 11.20 shoots and 12.38 leaves, respectively. Importantly, this same combination also demonstrated superiority across other vital growth parameters, including plant height (1.41 cm), root number (7.00), and root length (0.61 cm). These specific results provide invaluable practical data for developing efficient *in vitro* protocols for *Coelogyne* hybrid orchids, offering a substantial contribution to horticultural biotechnology. While the abstract provides compelling results, a complete manuscript would greatly benefit from an explicit discussion of the statistical significance of the differences observed between treatments, which is critical for validating the "optimal" claims. Furthermore, exploring the physiological mechanisms behind the synergistic effects of Kinetin and GA3 on *Coelogyne* growth would deepen the scientific understanding. Future work could also extend to evaluating the performance of these *in vitro* developed plantlets during the acclimatization phase. Nevertheless, this study makes a robust and practical contribution to orchid tissue culture, providing a clear pathway for enhancing the propagation of valuable *Coelogyne* hybrids.

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