Damasa Ines Larrisa, Pangesti Nugrahani, Sri Wiyatiningsih

THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO

Introduction

The role of agnps and ab mix in the growth of aglaonema 'lady valentine' in vitro & ex vitro. Enhance Aglaonema 'Lady Valentine' propagation. Discover how 2ppm AgNPs and 3ml/L AB Mix optimize in vitro growth, ex vitro acclimatization, and plantlet health. Boost multiplication.

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization

Review

This study tackles a critical challenge in ornamental horticulture: the efficient propagation of *Aglaonema* ‘Lady Valentine’, a plant renowned for its economic value, by addressing the limitations of conventional methods and enhancing existing tissue culture and acclimatization protocols. The authors systematically explored the combined influence of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on both in vitro and ex vitro plantlet development. This research provides a significant contribution by identifying specific treatment combinations that demonstrably improve propagation efficiency, thus offering a practical solution to current bottlenecks in *Aglaonema* production. The experimental design, a two-factorial completely randomized design with four levels and three replications, appears sound and well-suited to the study's objectives. The findings are particularly compelling, revealing a synergistic relationship between AgNPs and AB Mix. Specifically, the combination of 2 ppm AgNPs and 3 ml/L AB Mix consistently achieved superior in vitro performance, leading to increased plantlet height, volume, and overall visual health. This optimal treatment, along with 3 ppm AgNPs combined with 3 ml/L AB Mix, also proved highly effective during ex vitro acclimatization, promoting better plantlet adaptation, as evidenced by enhanced height, number of leaves and shoots, and seedling viability. These clear, quantifiable results offer valuable, actionable insights for commercial plant propagators. To further enrich the scope and impact of this research, future studies could delve into the precise physiological and molecular mechanisms through which AgNPs and AB Mix interact to stimulate growth and aid acclimatization. Investigating the long-term genetic stability and phenotypic fidelity of plantlets propagated under these optimized conditions would also be a crucial next step. Additionally, an economic feasibility analysis of incorporating AgNPs into large-scale *Aglaonema* production could provide valuable context for commercial adoption. Nonetheless, this study successfully establishes an effective strategy for improving *Aglaonema* propagation, laying a strong foundation for subsequent advancements in plant tissue culture.

Full Text

Comments

You need to be logged in to post a comment.