Eko Kusumawati, Fakhriyana Fakhriyana, Sapri Sapri

PROFIL METABOLIT SEKUNDER EKSTRAK ETANOL DAUN KATUK (Sauropus androgynus) DAN UJI IN-VITRO AKTIVITAS ANTIBAKTERI TERHADAP Staphylococcus aureus MENGGUNAKAN DIFUSI SUMUR

Introduction

Profil metabolit sekunder ekstrak etanol daun katuk (sauropus androgynus) dan uji in-vitro aktivitas antibakteri terhadap staphylococcus aureus menggunakan difusi sumur. Profil metabolit sekunder dan uji antibakteri ekstrak etanol daun katuk (Sauropus androgynus) terhadap Staphylococcus aureus. Hasil in-vitro menunjukkan penghambatan signifikan.

Abstract

Infeksi bakteri primer pada kulit sering disebabkan oleh Staphylococcus aureus. Bakteri ini dapat menyebabkan beberapa peradangan pada kulit, seperti bisul, impetigo, selulitis, dan mastitis. Salah satu tanaman obat yang berkhasiat mengobati bisul ialah daun katuk (Sauropus androgynus (L.) Merr.). Telah dilakukan pengujian aktivitas antibakteri ekstrak etanol daun katuk terhadap pertumbuhan bakteri Staphylococcus aureus ATCC 25923 secara in-vitro. Ekstraksi daun dilakukan dengan metode maserasi menggunakan pelarut etanol 95%. Ekstrak etanol kasar diencerkan dengan pelarut dimetil sulfoksida (DMSO) 1% hingga berkonsentrasi 60%, 70%, 80%, 90%, dan 100%. Penelitian ini dilakukan menggunakan metode difusi sumuran agar. Parameter yang diukur ialah besarnya diameter zona hambat yang terbentuk disekitar sumur. Digunakan DMSO 1% sebagai kontrol negatif dan tetrasiklin HCl 0,1% sebagai kontrol positif. Desain penelitian ini menggunakan RAL dengan 5 perlakuan dan 2 kontrol, serta dilakukan 3 kali pengulangan. Rata-rata diameter zona hambat yang terbentuk dengan perlakuan ekstrak berkonsentrasi 60%, 70%, 80%, 90%, dan 100% secara berurutan ialah 5,42; 5,83; 6,58; 7,00; dan 8,75 mm. Hasil analisis statistik menggunakan proGram SPSS versi 16.0 for windows menunjukkan bahwa ekstrak etanol daun katuk berpengaruh secara signifikan terhadap DMSO 1% dan tetrasiklin HCl 0,1% dalam menghambat pertumbuhan bakteri Staphylococcus aureus ATCC 25923 pada taraf kepercayaan 95%.

Review

This study presents an investigation into the in-vitro antibacterial efficacy of an ethanol extract derived from *Sauropus androgynus* (Katuk) leaves against *Staphylococcus aureus* ATCC 25923, a clinically relevant pathogen responsible for various skin infections. Utilizing the agar well diffusion method, the researchers assessed the inhibitory effects across a range of extract concentrations from 60% to 100%. The abstract reports a clear dose-dependent increase in the diameter of inhibition zones, with the highest concentration yielding an average zone of 8.75 mm. These findings offer preliminary scientific validation for the traditional use of Katuk leaves in treating conditions such as boils, suggesting its potential as a source for novel antimicrobial agents. The methodology described demonstrates several commendable aspects, including the use of a standardized bacterial strain (*S. aureus* ATCC 25923) and appropriate controls, namely 1% DMSO as a negative control and 0.1% Tetracycline HCl as a positive control. The experimental design, a Completely Randomized Design incorporating five treatment concentrations and two controls with three replications, coupled with statistical analysis using SPSS, enhances the reliability of the reported results. The systematic presentation of increasing inhibition zone diameters (ranging from 5.42 mm to 8.75 mm) directly correlating with higher extract concentrations provides valuable insight into the dose-response relationship of the extract's antibacterial activity. Despite these strengths, a significant incongruity exists between the study's title, "PROFIL METABOLIT SEKUNDER EKSTRAK ETANOL DAUN KATUK (Sauropus androgynus)...", and the information provided in the abstract. The title explicitly promises a secondary metabolite profile, yet the abstract contains no data or discussion pertaining to any phytochemical analysis. This omission is a substantial weakness, as identifying the active compounds is crucial for understanding the mechanism of action and for future drug development. Furthermore, while the abstract states a significant effect relative to Tetracycline HCl, it omits the actual inhibition zone diameter for the positive control, hindering a direct quantitative comparison of efficacy. Future work should critically address the promised phytochemical profiling, clearly define the nature of the extract concentrations (e.g., w/v of dry extract), and comprehensively report the results of all controls to provide a more complete and insightful evaluation of *Sauropus androgynus*'s antibacterial potential.

Full Text

Comments

You need to be logged in to post a comment.