Naveila Al Azizah, Alyatul Faizah, Efa Lusiana

POTENSI BUAH JUWET (Syzygium cumini) UNTUK MENINGKATKAN NILAI TITER ANTIBODI TIKUS JANTAN (Rattus norvegicus)

Introduction

Potensi buah juwet (syzygium cumini) untuk meningkatkan nilai titer antibodi tikus jantan (rattus norvegicus). Ekstrak juwet (Syzygium cumini) potensial sebagai imunomodulator. Studi menunjukkan dosis 150 mg/kg BB efektif tingkatkan titer antibodi tikus jantan.

Abstract

Kesehatan dan kekebalan tubuh merupakan aspek penting yang perlu diperhatikan, terutama di era pandemi saat ini. Kemampuan tubuh dalam melawan benda asing (patogen) dapat diukur melalui titer antibodi. Penelitian ini bertujuan untuk menguji potensi ekstrak buah juwet (Syzygium cumini) sebagai imunomodulator terhadap titer antibodi tikus jantan yang diinduksi sel darah merah sapi. Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas 4 kelompok dan 5 ulangan. Variabel bebas yang digunakan adalah ekstrak buah juwet (Syzygium cumini) dengan tiga dosis, yaitu 50 mg/kg BB, 100 mg/kg BB, dan 150 mg/kg BB, serta perlakuan Na CMC sebagai kontrol. Perlakuan dimulai pada hari ke-1 dan diberikan satu kali sehari selama 11 hari. Tiap kelompok hewan percobaan diinjeksikan secara intraperitoneal pada hari ke-1 dengan 0,5 ml sel darah merah sapi (SDMS) 2% dalam PBS sebagai antigen. Kontrol negatif yang digunakan adalah pemberian Na CMC. Hasil pengukuran menunjukkan bahwa nilai titer antibodi tertinggi terdapat pada kelompok perlakuan ekstrak etanol buah juwet dengan dosis 150 mg/kg BB, yaitu sebesar 2,98, diikuti oleh dosis 100 mg/kg BB dengan nilai titer antibodi sebesar 2,94, dan dosis 50 mg/kg BB.

Review

This manuscript presents a timely and relevant investigation into the immunomodulatory potential of *Syzygium cumini* (Juwet) fruit extract, a topic of significant interest in the current global health landscape. The study's objective was to assess the extract's effect on antibody titers in male *Rattus norvegicus* induced with sheep red blood cells, a standard method for evaluating humoral immune responses. The abstract clearly highlights a primary finding: a dose-dependent increase in antibody titer, with the highest value observed at the 150 mg/kg BW dose, suggesting a beneficial effect on the immune system. The experimental design, employing a Completely Randomized Design (RAL) with four groups and five replicates, appears sound for an *in vivo* study of this nature. The use of Na CMC as a negative control provides a baseline for comparison. However, the abstract could be strengthened by mentioning if a positive control (a known immunomodulator) was included, which would provide a comparative benchmark for the extract's efficacy. Furthermore, while numerical antibody titer values are provided, the units or the scale of measurement (e.g., log2 hemagglutination titer) are not specified, making it difficult to fully interpret the magnitude of the effect without additional context. The absence of explicit mention of statistical analysis also leaves a gap in understanding the significance of the observed differences between the doses. Overall, this research offers promising preliminary data indicating that *Syzygium cumini* fruit extract possesses properties that can enhance antibody production, thus suggesting its potential as a natural immunomodulator. The findings lay a valuable foundation for future investigations into the specific bioactive compounds responsible for this effect, their underlying cellular and molecular mechanisms, and potential applications in animal or human health. While the abstract provides a compelling overview, the full manuscript should furnish comprehensive methodological details, including the precise measurement units for antibody titers and robust statistical analysis to substantiate the reported conclusions for publication.

Full Text

Comments

You need to be logged in to post a comment.