Devi Octavia, Arnia Sari Mukaromah, Irfan Martiansyah, Mimin Mimin, S Ma'mun, Herman Rukmanto

Isolasi DNA tumbuhan hasil eksplorasi di Nusakambangan dengan metode kit di Laboratorium Treub, Kebun Raya Bogor

Introduction

Isolasi dna tumbuhan hasil eksplorasi di nusakambangan dengan metode kit di laboratorium treub, kebun raya bogor. Laporan isolasi DNA tumbuhan eksplorasi Nusakambangan di Kebun Raya Bogor menggunakan metode kit untuk konservasi genetik. Hasil menunjukkan keberhasilan parsial dengan tantangan teknis.

Abstract

Konservasi genetik merupakan suatu tindakan untuk menjaga, mendokumentasikan dan mengarsipkan spesimen tertentu dalam bentuk koleksi hidup maupun koleksi genom. Pendekatan molekuler sebagai alternatif dalam konservasi genetik, umumnya dilakukan dengan mengisolasi organ tertentu hingga diperoleh materi genetiknya. Tujuan penulisan artikel ini adalah untuk melaporkan hasil isolasi DNA dari beberapa sampel tumbuhan yang terdapat di Laboratorium Treub, Kebun Raya Bogor. Isolasi DNA mendukung metode konservasi genetik dari koleksi herbarium dan Bank Biji di Kebun Raya Bogor yang sudah ada sebelumnya. Metode isolasi DNA yang dilakukan berbasis pada penggunaan 2 kit DNA khusus untuk tumbuhan dengan protokol baku pada masing-masing kit. Adapun hasil isolasi DNA dari 32 sampel menunjukkan bahwa pita DNA yang muncul menjadi tanda dari keberhasilan konservasi genetik secara molekuler. Dari 29 yang diisolasi menggunakan Kit #1. Hanya 1 sampel yang muncul pita DNA. Kemudian 5 sampel lainnya berhasil terisolasi menggunakan Kit #2. Dari 32 sampel yang diisolasi, 13 sampel muncul pita dan 19 (tidak muncul pita) dengan variasi konsentrasi DNA. Hal tersebut diasumsikan beberapa kesalahan teknis penanganan dalam proses laboratorium.

Review

The article titled "Isolasi DNA tumbuhan hasil eksplorasi di Nusakambangan dengan metode kit di Laboratorium Treub, Kebun Raya Bogor" addresses a highly relevant topic in biological conservation. Genetic conservation, particularly through molecular approaches, is crucial for safeguarding biodiversity, and the effort to establish such a program at a prominent institution like the Bogor Botanical Gardens is commendable. The primary objective of this study, as outlined in the abstract, was to report on the success of DNA isolation from various plant samples collected from Nusakambangan, thereby supporting and complementing existing herbarium and seed bank collections. The methodology employed involved the use of two distinct commercial DNA isolation kits, following their respective standard protocols, to process 32 plant samples. The results presented indicate a mixed success rate: out of 29 samples processed with Kit #1, only one yielded a discernible DNA band, while five additional samples were successfully isolated using Kit #2. In total, 13 out of 32 samples showed successful DNA isolation, with the remaining 19 exhibiting no visible DNA bands and a variation in DNA concentration. The authors attribute these failures and the reported variation in DNA concentration to "beberapa kesalahan teknis penanganan dalam proses laboratorium" (some technical handling errors during the laboratory process). While the initiative to establish molecular genetic conservation at the Bogor Botanical Gardens is a strong point of this work, the reported success rate of approximately 40% (13/32) highlights significant challenges that warrant further investigation. The general attribution of failures to "technical errors" in the abstract, while acknowledging a potential issue, lacks specific detail regarding the nature of these errors or potential mitigation strategies. For a more robust contribution, future work should delve deeper into troubleshooting, potentially optimizing protocols for specific plant types that might contain high levels of secondary metabolites, and providing quantitative data on DNA concentration and purity (e.g., A260/280 ratios) for all isolated samples. Demonstrating the utility of the isolated DNA through subsequent molecular analyses (e.g., PCR amplification of specific markers) would further strengthen the impact of this foundational work. The findings, despite the challenges, lay important groundwork for developing a standardized and effective molecular conservation pipeline for the valuable flora of Indonesia.

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