Nor Hilyati, Muhammad Adnan, Iin Novita Nurhidayati Mahmuda, Roro Flaviana Bayu Astiyani, Olivia Bunga Putri, Denny Bimatama Pradita

Correlation of Neutrophil and Procalcitonin Levels with Multiorgan Damage Scores in an Escherichia coli-Induced Male Rat Sepsis Model

Introduction

Correlation of neutrophil and procalcitonin levels with multiorgan damage scores in an escherichia coli-induced male rat sepsis model. Examine the correlation of neutrophil and procalcitonin levels with multiorgan damage scores in an E. coli-induced male rat sepsis model. Higher levels are linked to greater organ injury severity.

Abstract

infection, which may lead to multiple-organ damage. Neutrophils and procalcitonin (PCT) are inflammatory biomarkers known to increase during sepsis and are suspected to be associated with the severity of organ injury. This study aimed to analyze the relationship between neutrophil and procalcitonin levels and multiple-organ tissue damage scores in a male rat sepsis model induced by intraperitoneal administration of Escherichia coli. This was a true experimental laboratory study using a post-test-only control group design involving nine male Rattus norvegicus rats divided into a control group, an E. coli-induced group receiving 1 × 10⁶ CFU/kg body weight, and an E. coli-induced group receiving 1.5 × 10⁶ CFU/kg body weight. Neutrophil levels were measured using a hematology analyzer, while PCT levels were determined using an Enzyme-Linked Immunosorbent Assay (ELISA). Multiple-organ damage was assessed through histopathological examination of the liver, kidney, spleen, heart, and lungs. Data were analyzed using Spearman’s correlation test. The results showed that neutrophil levels were significantly and positively correlated with damage scores of the liver (rs = 0.88; p = 0.002), spleen (rs = 0.90; p < 0.001), heart (rs = 0.92; p < 0.001), and lungs (rs = 0.91; p < 0.001). Procalcitonin levels were also significantly and positively correlated with damage scores of the liver (rs = 0.90; p < 0.001), spleen (rs = 0.87; p = 0.002), heart (rs = 0.85; p = 0.004), and lungs (rs = 0.88; p = 0.002). In conclusion, higher neutrophil and procalcitonin levels were associated with greater multiple-organ tissue damage in a male rat sepsis model induced by Escherichia coli.

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