Melania Citra Pertiwi, Anna Rakhmawati, Bernadetta Octavia, Tien Aminatun, Suhartini

Isolation and Identification of Profenofos Pesticide-Tolerant Bacteria from Agriculture Land in Sleman Regency

Introduction

Isolation and identification of profenofos pesticide-tolerant bacteria from agriculture land in sleman regency. Discover profenofos pesticide-tolerant bacteria from Sleman agricultural land. Study identifies 10 isolates (5 genera) with bioremediation potential.

Abstract

The intensive application of organophosphate pesticides containing profenofos as the active compound may lead to soil contamination and the emergence of pesticide-tolerant bacterial strains with potential for bioremediation. Comprehensive identification and characterization of these bacteria are crucial to support sustainable agricultural and environmental management practices. The aim of the study is to analyze the characteristics and identify indigenous bacteria tolerant to profenofos pesticide in Sleman agricultural land, DIY. This study is qualitative research using an exploratory-descriptive. Isolation was carried out by dilution to a level of 10-7 followed by a spread plate on Mineral Salt Medium (MSM) which was given with 100 ppm profenofos pesticide. Bacterial isolates were characterized and then identified using profile matching with reference genera. Based on the results of the study, it is found that 10 bacterial isolates tolerant to organophosphate pesticide with the active ingredient profenofos that have a similarity index of ≥70%. Based on the reference bacteria, 5 different genera are obtained, namely Paracoccus (D3, D8), Micrococcus (D5, D6, D9), Enterococcus (D2, D10), Arthrobacter (D1, D4), and Neisseria (D7). The bacterial isolates obtained have different characteristics. The characteristics of these bacteria are gram positive and negative bacteria with round, irregular, and rhizoid shapes. Their margins are lobate, curled, entire, undulate, and ciliate. Their elevations are umbonate, raised, and flat. The color of the bacteria is white to light orange, with various physiological test results.

Review

This study tackles a highly relevant environmental issue concerning the intensive use of organophosphate pesticides like profenofos, which contributes to soil contamination and necessitates effective bioremediation strategies. The authors clearly state their aim to isolate and identify indigenous profenofos-tolerant bacteria from agricultural land, a critical first step in harnessing microbial potential for environmental cleanup. The successful isolation of 10 bacterial strains, identified across five distinct genera with a reported similarity index of ≥70%, demonstrates the presence of a diverse microbial community capable of tolerating this specific pesticide, laying foundational groundwork for future bioremediation efforts. The methodological approach, involving dilution and spread plating on Mineral Salt Medium supplemented with 100 ppm profenofos, appears appropriate for the selective isolation of tolerant strains. The subsequent characterization, including Gram staining, detailed morphological descriptions (shape, margin, elevation, color), and various physiological tests, provides a comprehensive phenotypic profile for each isolate. The identification of diverse genera such as *Paracoccus*, *Micrococcus*, *Enterococcus*, *Arthrobacter*, and *Neisseria* is a significant finding, highlighting the breadth of microbial adaptation to xenobiotic compounds in agricultural environments. These detailed phenotypic data are valuable for distinguishing the isolates and understanding their basic biological characteristics. While the study effectively isolates and characterizes profenofos-tolerant bacteria, its findings could be further strengthened by incorporating molecular identification techniques, such as 16S rRNA gene sequencing, to confirm the identified genera with higher confidence. Additionally, for these isolates to be truly valuable for bioremediation applications, future research should critically assess their actual profenofos *degradation capabilities*, beyond mere tolerance. Investigating the degradation pathways, kinetics, and the nature of the degradation products would provide crucial insights into their potential efficacy. Nevertheless, this work successfully identifies a promising collection of profenofos-tolerant bacteria, offering a valuable starting point for advanced studies in pesticide bioremediation and sustainable agricultural management.

Full Text

Comments

You need to be logged in to post a comment.