Sugireng Sugireng, Rosdarni Rosdarni

Deteksi gen nuc isolat bakteri Staphylococcus aureus dari pasien ulkus diabetikum dengan metode PCR

Introduction

Deteksi gen nuc isolat bakteri staphylococcus aureus dari pasien ulkus diabetikum dengan metode pcr. Identifikasi gen nuc Staphylococcus aureus pada pasien ulkus diabetikum menggunakan metode PCR. Penelitian ini mendukung diagnostik dini S. aureus penyebab infeksi luka diabetes.

Abstract

Bakteri Stapylococcus aureus adalah salah satu bakteri yang dapat menyebabkan infeksi pada berbagai jaringan tubuh pada penderita ulkus diabetikum. Meluasnya infeksi S. aureus, maka alternatif diagnostik dini spesifik S. aureus sangat dibutuhkan. Salah satu teknik diagnostik yang cepat dan akurat yaitu dengan menggunakan PCR (polymerase chain reaction) dengan mendeteksi gen spesifik S. aureus yaitu gen nuc. Penelitian ini bertujuan untuk mendeteksi adanya gen nuc yang merupakan gen pengkode produksi Tnase (Thermonuclease) bakteri S. aureus yang menginfeksi luka pada pasien diabetes. Penelitian ini dilakukan dengan 3 tahapan yaitu pengambilan sampel swab luka pasien ulkus diabetikum, proses isolasi DNA, proses PCR dan elektroforesis serta didokumentasikan dengan UV transluminator. Berdasarkan hasil penelitian dihasilkan dari 2 sampel isolat bakteri pasien ulkus diabetikum yaitu sampel 1 dan sampel 4. Kedua sampel memiliki gen nuc yang ditandai dengan terbentuk pita DNA ukuran 278 bp.

Review

The study "Deteksi gen nuc isolat bakteri Staphylococcus aureus dari pasien ulkus diabetikum dengan metode PCR" addresses a critical issue in clinical microbiology: the rapid and specific diagnosis of *Staphylococcus aureus* infections, particularly in the context of diabetic foot ulcers. *S. aureus* is a significant pathogen in these patients, and early, accurate detection is crucial for effective treatment. The authors propose the use of PCR to detect the *nuc* gene, which codes for thermonuclease, as a specific diagnostic marker for *S. aureus*. This approach is highly relevant given the urgent need for swift and precise diagnostic tools in managing complex and potentially limb-threatening infections. The methodology described is straightforward, involving the collection of wound swab samples from diabetic ulcer patients, subsequent DNA isolation, PCR amplification targeting the *nuc* gene, and visualization via gel electrophoresis and UV translumination. The abstract reports a positive finding in two specific isolates (Sample 1 and Sample 4) from diabetic ulcer patients. For these two samples, the presence of the *nuc* gene was confirmed by the detection of a distinct DNA band at 278 bp, consistent with the expected amplicon size. This demonstrates the technical feasibility of using PCR for *nuc* gene detection in clinical isolates. While the study successfully demonstrates the principle of *nuc* gene detection by PCR in a clinical setting, a significant limitation is the extremely small sample size, with results presented for only two isolates. This small number precludes any generalizable conclusions regarding the prevalence or diagnostic utility of this method across a broader patient population. To enhance the impact and clinical relevance of this work, future research should involve a substantially larger cohort of patients and *S. aureus* isolates. Additionally, it would be beneficial to include a comparison with conventional microbiological identification methods to validate the PCR findings further. Despite its preliminary nature, this study lays foundational groundwork for utilizing PCR-based *nuc* gene detection as a rapid diagnostic tool for *S. aureus* in diabetic ulcer infections, provided it is expanded and rigorously validated.

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