Rakhmadi I., Muladno Muladno, H. C. H. Siregar, Pollung H. Siagian

PERFORMANCE OF MALE MICE (MUS MUSCULUS) AGE 28-63 DAYS IN THE COTE WITH HUSK LAYER, SAND LAYER, AND ZEOLITE LAYER WITH PARTITION OR NOT

Introduction

Performance of male mice (mus musculus) age 28-63 days in the cote with husk layer, sand layer, and zeolite layer with partition or not. Evaluate male mice (28-63 days) performance in cotes using husk, sand, or zeolite layers, with/without partitions. Examines feed intake, growth, mortality, and ammonia levels for optimal housing.

Abstract

The study aimed to analyze the effect of different layer types with pertition or not against perfomence male mice ages 28-63 days and to find out the information of ammonia on different layer cotes. The parameters measured were feed intake BK, dringking water, beginning and ending BW, ADG, feed convertions, mortality, and ammonia on cages. CRD designs was used in 3 x 2 factorial experiment, the first factor are types of layer (huks, sand, and zeolite) and the seconds factor are partition of layer (without insulation and section). The data obtained were analyzed with ANOVA if there are a real result, followed by Tukey test appeal. The data which not suitabele with the parameters was analyzed with Kruskal-Wallis test, whereas the ammonia on cote described descriptively. The result showed that different types of layer most significant (p

Review

This study addresses a relevant topic in laboratory animal science, investigating the impact of different bedding materials and cage partitions on the performance and welfare of male mice. Optimizing housing conditions is crucial for maintaining animal health, ensuring experimental reproducibility, and improving overall resource efficiency in research and breeding facilities. The clear aim to analyze the effects of layer types and partitions on multiple performance parameters, alongside measuring ammonia levels, is well-defined and contributes practical knowledge to animal husbandry. The methodology, employing a 3x2 factorial Completely Randomized Design, is appropriate for systematically evaluating the independent and interactive effects of the chosen factors (husk, sand, zeolite layers; with or without partition). The inclusion of a comprehensive range of performance parameters, such as feed intake, water intake, body weight changes, average daily gain, feed conversion, and mortality, allows for a holistic assessment of animal well-being and productivity. Furthermore, the measurement of ammonia levels is a critical addition, as it directly relates to air quality and can significantly impact respiratory health and stress in rodents. The planned statistical analysis, utilizing ANOVA, Tukey's test, and Kruskal-Wallis where appropriate, indicates a sound approach to data interpretation. However, the abstract contains several typographical errors and grammatical inconsistencies (e.g., "pertition," "perfomence," "dringking water," "feed convertions," "huks," "seconds factor"), which should be meticulously corrected in the full manuscript to enhance clarity and professional presentation. While the abstract concludes abruptly, stating that "different types of layer most significant (p...", it clearly points to a primary finding where the choice of bedding material significantly influences mouse performance. The full paper should provide detailed results for each performance parameter, explicitly stating which layer types performed optimally under which partition conditions, and quantitatively present the ammonia findings. Elucidating the specific mechanisms by which different layers and partitions affect mouse physiology and behavior would greatly strengthen the discussion. The implications of these findings are significant for improving animal welfare, standardizing housing conditions in research, and potentially informing guidelines for laboratory animal care. A thorough proofread and native English speaker review of the entire manuscript is highly recommended before submission to ensure accuracy and professionalism.

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