Sang Gede Purnama

DETECTING TRANSOVARIAL INFECTION IN AEDES AEGYPTI BASED ON IMMUNOCYTOCHEMICAL STREPTAVIDIN BIOTIN PEROXIDASE COMPLEX ASSAY (ISBPC) IN BALI

Introduction

detecting transovarial infection in aedes aegypti based on immunocytochemical streptavidin biotin peroxidase complex assay (isbpc) in bali. Detect transovarial dengue virus in Aedes aegypti mosquitoes in Bali using ISBPC. This study explores the link between mosquito-to-egg transmission and DHF incidence in Bali.

Abstract

Immunocytochemical method is one of dengue virus examination alternative with affordable cost. Through immunocytochemical methods it will be proven that transovarial transmission of dengue virus from mosquitoes to their eggs and its relationship with the incidence of DHF in Bali. This study is done by the installationovitrap indoor and outdoor as much as 1200 points. The mosquito will be cultured for dengue virus examination using the Immunocytochemical-Immunomodoxidase streptavidin biotin complex (IISBC) method of head squash. The results of examination of larvae density were known to the ovitrap index in Denpasar (4.4), Tabanan (6.6), Gianyar (8.9). There is evidence of transovarial transmission from female mosquitoes to eggs using Immunocytochemical Technique known to its transovarial index of each city ie Denpasar (11%), Gianyar (7.14%) and Tabanan (7.14%). Key words: transovarial, immunocytochemical, Bali

Review

This study presents an investigation into the detection of dengue virus transovarial infection in *Aedes aegypti* mosquitoes in Bali using an immunocytochemical streptavidin biotin peroxidase complex (ISBPC) assay. The research aims to establish the presence of transovarial transmission and its potential link to Dengue Hemorrhagic Fever (DHF) incidence, offering an affordable alternative diagnostic method. The main findings include varying ovitrap indices across Denpasar (4.4), Tabanan (6.6), and Gianyar (8.9), and critically, the successful detection of transovarial transmission with respective transovarial indices of 11% in Denpasar and 7.14% in Gianyar and Tabanan. This work is relevant for understanding dengue epidemiology and informing control strategies in endemic areas like Bali. While the study presents an interesting and potentially cost-effective approach to detecting dengue virus in mosquito populations, several aspects require significant clarification. Firstly, there is an inconsistency in the assay name, alternating between "ISBPC" and "IISBC" in the abstract, which should be standardized. More critically, although the abstract explicitly states an objective to examine the "relationship with the incidence of DHF in Bali," no results pertaining to this crucial correlation are presented, leaving a notable gap between the stated aim and the reported findings. Methodologically, the description of "head squash" examination and "culturing" lacks sufficient detail; it is unclear whether adult mosquitoes or their offspring (eggs/larvae) were directly tested for transovarial infection, which is central to the study's premise. Furthermore, while transovarial indices are reported as percentages (e.g., 11%), the total number of tested eggs or larvae from which these percentages were derived is not provided, making it difficult to assess the statistical robustness of these figures. A discussion on the validation of the ISBPC method against a gold standard (e.g., RT-PCR) for detecting DENV in mosquito tissues would also significantly strengthen the work. Despite these points requiring clarification, the study offers valuable preliminary insights into the prevalence of transovarial dengue transmission in *Aedes aegypti* populations in Bali. The use of an immunocytochemical method for this purpose is commendable for its potential affordability and accessibility in resource-limited settings, offering a practical tool for vector surveillance. To maximize its impact and ensure scientific rigor, future iterations of this work should address the identified methodological ambiguities, clearly articulate findings related to DHF incidence if it remains an objective, and provide more comprehensive data on sample sizes and assay validation. Such refinements would solidify the contribution of this research to our understanding of dengue ecology and vector control efforts.

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