lisna dewi, Tuty Slamet, Neneng Zianida

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN KENTANG HITAM (Plectranthus rotondifolius (Poir) Spreng) DENGAN METODE DPPH (2-2-difenil-1-pikrilhidrazil)

Introduction

Aktivitas antioksidan ekstrak etanol daun kentang hitam (plectranthus rotondifolius (poir) spreng) dengan metode dpph (2-2-difenil-1-pikrilhidrazil). Uji aktivitas antioksidan ekstrak etanol daun kentang hitam (Plectranthus rotondifolius) via metode DPPH. Hasilnya sangat kuat dengan IC50 14,6 µg/ml.

Abstract

Kentang merupakan tanaman yang memiliki kemampuan sebagai antioksidan. Senyawa metabolit sekunder yang terkandung dalam kentang diantaranya adalah alkaloid, flavonoid, saponin, tanin, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antioksidan ekstrak etanol daun kentang hitam dengan menggunakan metode DPPH. Karakterisasi simplisia didapatkan hasil kadar air sebesar 2,4%, susut pengeringan sebesar 6,5%, kadar abu total sebesar 12,5%, kadar abu tidak larut asam sebesar 0,5%, kadar sari larut air sebesar 32%, dan kadar sari larut etanol sebesar 21%. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol 70% dengan hasil rendemen sebesar 13%. Uji aktivitas antioksidan menggunakan metode penghambatan radikal bebas DPPH (2,2-difenil-1-pikrilhidrazil). Hasil penelitian menunjukkan nilai IC50 ekstrak etanol daun kentang hitam sebesar 14,6 µg/ml dan nilai IAA sebesar 2,73, sehingga ekstrak etanol daun kentang hitam memiliki aktivitas antioksidan yang sangat kuat.

Review

This study presents an investigation into the antioxidant potential of *Plectranthus rotondifolius* (Black Potato) leaf extract, a plant noted for its content of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, and phenols. The overarching aim was to quantify the antioxidant activity of its ethanol extract using the widely accepted DPPH radical scavenging method. Given the increasing interest in natural sources for antioxidants, this research is timely and addresses a relevant area of phytopharmacology, offering insights into a potentially valuable plant material. The methodology employed rigorous initial characterization of the simplisia, reporting critical parameters such as water content (2.4%), drying shrinkage (6.5%), total ash (12.5%), acid-insoluble ash (0.5%), water-soluble extract (32%), and ethanol-soluble extract (21%). The maceration extraction process, utilizing 70% ethanol, yielded a 13% extract. The core finding, however, lies in the antioxidant assay results: an impressive IC50 value of 14.6 µg/ml and an IAA value of 2.73. These quantitative results unequivocally classify the ethanol extract of black potato leaves as possessing very strong antioxidant activity, which is a significant outcome for the plant's potential therapeutic applications. Overall, the study successfully establishes the potent antioxidant capacity of *Plectranthus rotondifolius* leaf extract, providing a strong foundation for further research. While the abstract clearly outlines the promising results and robust initial characterization, future work could benefit from linking the observed antioxidant activity more directly to specific classes of identified secondary metabolites. Additionally, expanding the scope to include other antioxidant mechanisms, *in vitro* and *in vivo* studies, and a comparison with established synthetic antioxidants would further strengthen the scientific contribution. Nevertheless, this paper presents compelling evidence for the very strong antioxidant activity of black potato leaves, highlighting its potential as a source of natural antioxidants.

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