Nadia Osama, Pina Budiarti Pratiwi, Dikki Miswanda

Optimasi Konsentrasi Perak Nitrat dalam Sintesis Nanopartikel Perak Berbasis Ekstrak Daun Jarak Pagar untuk Aplikasi Antibakteri dalam Teknologi Nano

Introduction

Optimasi konsentrasi perak nitrat dalam sintesis nanopartikel perak berbasis ekstrak daun jarak pagar untuk aplikasi antibakteri dalam teknologi nano. Sintesis nanopartikel perak green synthesis dari ekstrak daun jarak pagar. Optimasi konsentrasi perak nitrat untuk aplikasi antibakteri efektif dalam teknologi nano.

Abstract

Penelitian ini membahas tentang sintesis nanopartikel perak secara green synthesis melalui penggunaan ekstrak daun jarak pagar (Jatropha curcas L.) sebagai reduktor dan larutan perak nitrat (AgNO₃) sebagai prekursor dengan variasi konsentrasi 1, 2, 3, dan 4 mM. Tujuan utama penelitian adalah untuk menilai pengaruh variasi konsentrasi AgNO₃ terhadap ukuran nanopartikel dan aktivitas antibakterinya. Karakterisasi nanopartikel dilakukan melalui spektroskopi UV-Vis untuk memahami sifat kimia dan Particle Size Analyzer (PSA) untuk mengukur ukuran partikel. Pengukuran UV-Vis menunjukkan bahwa puncak serapan nanopartikel perak berada pada kisaran panjang gelombang 423–435 nm. Pengukuran ukuran partikel dengan PSA mengungkapkan bahwa ukuran partikel perak yang terbentuk berkisar antara 169–2949 nm, ukuran paling stabil diperoleh pada konsentrasi AgNO₃ sebesar 1 mM. Pengujian aktivitas antibakteri dilakukan dengan metode difusi cakram Kirby-Bauer terhadap bakteri Staphylococcus aureus. Hasilnya menunjukkan bahwa nanopartikel perak dengan konsentrasi AgNO₃ 1 mM memberikan zona hambat yang paling jelas dan stabil.

Review

This study presents a timely and relevant investigation into the green synthesis of silver nanoparticles, utilizing *Jatropha curcas L.* leaf extract as a natural reducing agent. The research addresses a critical aspect of nanoparticle synthesis by systematically optimizing the concentration of silver nitrate precursor, which directly impacts the physicochemical properties and biological activity of the resulting nanoparticles. The objective to evaluate the influence of varying AgNO₃ concentrations on both nanoparticle size and antibacterial efficacy is well-defined, promising significant contributions to the field of sustainable nanotechnology and antimicrobial applications. The methodology involved the synthesis of silver nanoparticles across a range of AgNO₃ concentrations (1, 2, 3, and 4 mM). Characterization was appropriately conducted using UV-Vis spectroscopy, revealing characteristic silver nanoparticle absorption peaks between 423–435 nm. Particle Size Analyzer (PSA) measurements indicated a broad particle size range from 169–2949 nm, with the abstract highlighting that the most stable particle size was achieved at 1 mM AgNO₃. Antibacterial activity was assessed against *Staphylococcus aureus* using the Kirby-Bauer disc diffusion method. Notably, the nanoparticles synthesized with 1 mM AgNO₃ demonstrated the most distinct and stable zone of inhibition, correlating optimal precursor concentration with superior antibacterial performance. Overall, this research offers a valuable contribution by demonstrating an effective green synthesis route for silver nanoparticles using a readily available plant extract, and by establishing a clear correlation between precursor concentration and both particle characteristics and antibacterial efficacy. A minor point for enhancement would be to explicitly quantify the "most stable size" obtained at 1 mM AgNO₃, given the wide overall size range reported, as this would further solidify the claims regarding optimization. Nevertheless, the findings regarding enhanced antibacterial activity at the optimized concentration are compelling and underscore the potential of these biogenic nanoparticles for various antibacterial applications within nanotechnology. The study provides a solid foundation for further investigations, potentially including mechanistic studies or broader spectrum antimicrobial testing.

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